BCECF-AM介绍
BCECF-AM is a cell membrane permeable compound widely used as a fluorescent indicator for intracellular pH. BCECF-AM could diffuse through the cell membrane and intracellular esterase cleave the ester bond releasing BCECF (HY-101882). BCECF allows measurements in the physiological pH range 6.0-8.0. Excitation ratio: 490/440 nm; Emission intensity: 535 nm.
BCECF AM与BCECF的关系
BCECF 本身是水溶性的，无法穿透细胞膜；而BCECF AM引入了乙酰氧基甲酯（AM）基团，增强了疏水性，可被动扩散进入细胞。胞内的酯酶会水解AM基团，生成滞留于胞内的BCECF，从而实现对细胞内pH的特异性检测。
BCECF-AM是什么
中文名称：2',7'-二-(2-羧乙基)-5(6)-羧基荧光素乙酰甲酯
英文名称：BCECF-AM；BCECF Acetoxymethyl ester
CAS号：117464-70-7
分子式：C40H36O19
分子量：820.7
Ex(nm)：450
Em(nm)：531
溶剂：DMSO
纯度：≥95%
外观：固体
结构式：
规格：1mg，5mg，10mg，包装可定制
产地：西安
储存：推荐长期储存标准为-20℃，并置于干燥避光环境以防潮解或变质
参考文献
1. Simultaneous measurement of water volume and pH in single cells using BCECF and fluorescence imaging microscopy
Authors: Alvarez-Leefmans FJ, Herrera-Perez JJ, Marquez MS, Blanco VM.
Journal: Biophys J (2006): 608
2. Photophysics of the fluorescent pH indicator BCECF
Authors: Boens N, Qin W, Basaric N, Orte A, Talavera EM, Alvarez-Pez JM.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2006): 9334
3. Drug efflux transport properties of 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (bcecf-am) and its fluorescent free acid, bcecf
Authors: Bachmeier CJ, Trickler WJ, Miller DW.
Journal: J Pharm Sci (2004): 932
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